BT

Culture Medium BT (for marine algae, except diatoms)

Final concentrations of additions and preparation of stock solutions:

 

final concentration

Trace elements

Mol L-1

Na2EDTA

5x10-6

Fe-(III)-citrate

2,5x10-6

MnCl2x4H2O

5x10-7

ZnSO4x7H2O

5x10-8

Na2MoO4x2H2O

2,5x10-9

CoCl2x6H2O

5x10-9

CuSo4x5H2O

5x10-10

H2SeO3

5x10-10

NaVO3

5x10-10

NiSO4x6H2O

5x10-10

 

 

Nutrients

 

Na NO3

4,42x10-4

Na2glyceroPO4

5x10-5

NaH2PO4

1,81x10-5

NH4Cl

2,5x10-5

 

 

Buffer

 

TRIS pH 7,2

1x10-3

 

 

Vitamins

 

Thiamine

3x10-7

Biotin

2,1x10-9

Vitamin B12

3,7x10-10

 Preparation of stock solutions: